Today, liquid chromatographic methodologies are used for a wide range of applications. It is firmly important in clinical investigations, and provides an intensive detection of particular sections that are effective enough to cure any illness. Liquid chromatography techniques, in particular, can aid in the testing and filtration of pharmaceutical treatments, which is beneficial to the development of the food and medical industries. Improved liquid chromatography means are urgently needed for increased argument of this process to value lives. This paper discusses the use of liquid chromatography to determine the presence of parabens and other preservatives in cosmetics and personal cosmetic products.
Definition of Liquid Chromatography
Liquid chromatography is an analytical chromatographic process that is convenient for separating compounds that are dissolved in a given solvent. Instrumentation Simple liquid chromatography contains a column that holds a stationary phase in equilibrium with a solvent. Typical stationary phases include solids for adsorption, ionic groups acting on a resin for ion exchange, liquids for partitioning, and porous inert particles. The mixture to is placed on the top of the column and more solvent is added. The varying components in the sample mixture pass through the column at diverse rates due to changes in their partitioning performance between the mobile liquid phase and the stationary phase. The mixtures are divided by gathering aliquots of the column overflow as a function of time.

Definition of Parabens

Paraben is a group of mixtures applied as preservers in medical and cosmetic products and in the nutrition production.

Artificially, they are a progression of esters of benzoic acid. Parabens are absorbing presevatives in variety of equations. (Kenneth A. Connors, Gordon L. Amidon and Valentino J. Stella)

Determination of Parabens and Preservatives in Cosmetics and Personal Cosmetic Products by Liquid Chromatography

Cosmetics contains water require protection against the development of microorganisms to provide product health. Preservatives are normal, microbial development, unfortunate chemical changes, or to expand the products timeframe of realistic usability. The application of preservative in individual cosmetic products is important because not exclusively do they prevent item harm caused by microorganisms however, they likewise help shield the item from coincidental defilement by the customer amid utilize. (Salvatore Fanali, Paul R. Haddad and Colin Poole) Without the expansion of preservative, the item may wind up plainly sullied, which can prompt item debasement and, because of restorative establishments, at last increment the danger of aggravation or even disease. Preservatives are added to personal cosmetic products at generally low levels to offer products, which are safe and long lasting. Determination of preservatives in these items is essential for quality control to prevent unfavorably susceptible reactions and other medical problems.

The most broadly applied preservative in corrosive products are a class of mixes by and large alluded to as parabens. The compounds are alkyl esters of p-hydroxybenzoic corrosive. They are used for their preservative properties in corrosive and personal cosmetic items due to their antimicrobial exercises, low poisonous quality, and low generation cost. Methylparaben (MeP) is found in about all beauty cosmetic products and numerous pharmaceuticals. The application of parabens in personal cosmetic items has caused concern because of their potential antagonistic impacts, including growth of breast cancer and decline of sperm tally and testosterone levels. The United States Food and Drug Administration (FDA) finds that in spite of the fact that parabens can emulate estrogen, the levels found in these items are at such low levels that their movement on the body does not cause tumor in any higher rate than normally happening estrogen in spite of opposite belief. Be that as it may, when fake items make it to showcase, they are unregulated and may contain additive levels that may represent a health danger to the client. In this manner, exact procedures to decide the levels of these compounds should be accessible for checking preservative fixations in restorative and personal cosmetic items. (M. Silvia Díaz‐Cruz and Damià Barceló)

Figure 1: Chemical structure of paraben

Current technique for the determination of preservatives cosmetics and individual cosmetic items involve of high performance liquid chromatography, ultrahigh-pressure liquid chromatography, couple mass spectrometry, gas chromatography and mass spectrometry, strong stage microextraction and micellar electrokinetic chromatography (Michigan).


A liquid chromatography technique has been created to decide various preservatives in cosmetic and individual cosmetic products. The research is constantly short and straightforward and when combined with the ideal chromatographic conditions, the process takes into account short time for analysis. Contrasted with other scholarly references, Liquid chromatograph method combines a basic and simple trial research process along with a short investigation time while giving comparable if not better separation and cosmetic.

Works Cited

Kenneth A. Connors, Gordon L. Amidon and Valentino J. Stella. Chemical Stability of Pharmaceuticals: A Handbook for Pharmacists. John Wiley & Sons, 2011.

M. Silvia Díaz‐Cruz and Damià Barceló. Personal cosmetic Products in the Aquatic Environment. Springer, 2015. Document.

Michigan, The University of. LC GC: Magazine of Liquid and Gas Chromatography, Volume 5, Pages 1-529. Michigan: Aster Publishing Corporation, 2010.

Pryde, S. and M.T Gilbert. Applications of High Performance Liquid Chromatography. 4th Edition. London: Springer Science & Business Media, 2017. Print.

Salvatore Fanali, et al. Liquid Chromatography: Applications. Newnes, 2013. Print.

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